intraperitoneal injection in humans

The supernatants were diluted 1:30 with assay buffer consisting in 50mM phosphate buffer pH 6.0 with 0.167mg/mL o-dianisidine (Sigma) and 0.0005% H2O2.The colorimetric reaction was measured at 450nm between 1 and 3min in a spectrophotometer (Varioskan Flash Multimode Reader, Waltham, MA). Intraperitoneal Route of Drug Administration: Should it Be Used in Journal of immunology (Baltimore, Md. Article Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Lopez-Cubero, S. O., Sullivan, K. M. & McDonald, G. B. Article Best way for mesenchymal stem cells transplantation for colitis. In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. Clinical and experimental immunology 116, 238245 (1999). Direct injection of sodium pentobarbital (referred to as euthanasia by . Twenty-five Kunming mice were exposed to Fe 3 O 4-nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. 2A), which guarantee the less interference from the background signal that coming from free dye. Models should begin with local tissue infection, so that bacterial . : 1950) 180, 15981608 (2008). B.X. Drug Deliv. Little is known about neuronal cell death in mouse models of diabetic retinopathy. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. 2, Haidian District, Beijing 100193, China. Then, the bone marrow was flushed out with 1mL syringe. Murine Responses to Endotoxin: Another Dirty Little Secret? The medium was refreshed every 23 days and third- and fourth-passage cells were used for all experiments. We believe with the increasing interests for IP injection in the field of cytotherapy, more innovation and creative ideas for reducing IP complications will come up. Just mentioned above, immune cells and MSCs interaction in aggregates promotes TSG-6 secretion and contributed to the colitis recovery. This study included unilateral SSNHL patients who were hospitalized in our department between . Int J Stem Cells 1, 17 (2008). Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Article Macrophage Reverse Cholesterol Transport | Circulation Severity of DSS-induced colitis was also assessed using a score system by two independent pathologists. IP injections were suitable for serial imaging as well as imaging mice at multiple time points, which would be ideal for longitudinal drug treatment experiments. Castelo-Branco, M. T. et al. The AI and IV showed no significant intergroup difference, though AI had lower score (Fig. Acceptable Method (s) Injected barbiturates and barbiturate combinations. Fresh feces from animals were collected for occult blood test, using fecal occult blood kit (Baso Diagnostics Inc., Zhuhai, China) according to the manufactures recommendations. DailyMed - PIPERACILLIN AND TAZOBACTAM injection, powder, lyophilized Sci. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. The GFP+ MSCs were found at inflamed colon in both IP and AI groups 24h after MSCs injection. Their findings showed that intraperitoneally injected MSCs would form aggregates with macrophages and lymphocytes in peritoneal cavity and remotely secreted TSG-6. designed the research. 3) and the IV group preserved fewer cell number. Shariati M, Zhang H, Van de Sande L, Descamps B, Vanhove C, Willaert W, Ceelen W, De Smedt SC, Remaut K. Pharm Res. Intraperitoneal injection is widely used in rodents as a route of drug administration. The histological score was defined as the sum of the two parameters (total score=E+I). We have proposed that safe and effective protection against the development of adult onset cancers may be achieved by vaccination against tissue-specific self-proteins that are "retired" from expression at immunogenic levels in normal tissues as we age, but are overexpressed in emerging tumors. Overall, many studies utilize IP injections to deliver therapeutics to lab animals due to the efficiency of the administration route. These findings may benefit clinic practice. Horwitz, E. M. et al. Two patients with long-standing ulcerative colitis, psoriasis and leukemia underwent allogeneic stem cell transplantation and all the three disorders were in clinical remission for 4 years after transplantation11. International journal of molecular medicine 37, 115125, doi: 10.3892/ijmm.2015.2413 (2016). However, for IV injection, we and others found most mice will lead increased mortality because of potential pulmonary cell embolus when the cell dose increase to 110722. The https:// ensures that you are connecting to the The excitation/ emission spectrum of DiR is in the near infrared range (excitation 750nm and emission 782nm). Wang, N. et al. Mesenchymal Stem Cells Reduce Colitis in Mice via Release of TSG6, Independently of Their Localization to the Intestine. Article Background. Intraperitoneal chemotherapy for peritoneal metastases: an expert opinion. Google Scholar. Stem Cell Res Ther 3, 51, doi: 10.1186/scrt142 (2012). Available at: http://ctep.cancer.gov/highlights/20060105_ovarian.htm (Accessed: 7th April 2016) (2006). If injecting a hazardous substance (biohazard, radiation hazard or chemical hazard), include precautions in the Animal Care Protocol for human safety (e.g. Intraperitoneal injection of a substance in the mouse. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. A novel intraperitoneal metastatic xenograft mouse model for - PLOS Free DiR and healthy mice (without colitis) were served as control. Article The authors declare no competing financial interests. In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. Curr Opin Gastroenterol 30, 8496, doi: 10.1097/MOG.0000000000000031 (2014). The immunohistochemistry indicated higher repair proliferation (Ki-67) and more FoxP3+ cells accumulation of IP group. Comparison of colonic FoxP3 expression 7 days after MSCs injection. T1DM was induced by intraperitoneal injection of STZ (Sigma-Aldrich, St. Louis, MO, USA) in 25 mM sodium citrate (pH 4.5), with the dosage of 200 mg/kg body weight, into 10-week-old BALB/c mice with the body weight of 2325 g. . Li, C. Y. et al. In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. Google Scholar. IHC was performed according to the standard procedures. Ballinger AE, Palmer SC, Wiggins KJ, Craig JC, Johnson DW, Cross NB, Strippoli GF. [5] In 1967, the production of amnesia was studied through an injection of physostigmine. The following approaches are novel, and require further testing in humans before being accepted as potential treatment options: Saroglitazar suppresses the hepatocellular carcinoma induced by In another Phase I clinical trial, patients with ovarian cancer were injected intraperitoneally with dl1520 in order to study the effects of a replication-competent/-selective virus. n=3 per group. We notice that the mean density of collagen was lowest in IP group, however, IP group did not exhibit less collagen deposition than the AI group, probably due to the scatter points (Fig. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. PubMed PubMed Central In humans, numerous plasma molecules prevent or modulate cell stimulation by microbial products, either by neutralizing the agonists (eg, bactericidal permeabilityincreasing protein, . Currently, the intravenous injection (IV) is historically most common methods for MSCs delivery12. The fate of human peripheral blood lymphocytes after - PubMed CAS Goncalves Fda, C. et al. Therapeutic effects of a single injection of human umbilical mesenchymal stem cells on acute and chronic colitis in mice, Mechanism and therapeutic effect of umbilical cord mesenchymal stem cells in inflammatory bowel disease, Human induced pluripotent stem cell-derived mesenchymal stem cells promote healing via TNF--stimulated gene-6 in inflammatory bowel disease models, Conditioned medium of mesenchymal stem cells pretreated with H2O2 promotes intestinal mucosal repair in acute experimental colitis, Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis, Supporting the gastrointestinal microenvironment during high-dose chemotherapy and stem cell transplantation by inhibiting IL-1 signaling with anakinra, The recruitment of extra-intestinal cells to the injured mucosa promotes healing in radiation enteritis and chemical colitis in a mouse parabiosis model, Prolyl hydroxylase 2 silencing enhances the paracrine effects of mesenchymal stem cells on necrotizing enterocolitis in an NF-B-dependent mechanism, Challenges and opportunities targeting mechanisms of epithelial injury and recovery in acute intestinal graft-versus-host disease, http://ctep.cancer.gov/highlights/20060105_ovarian.htm, http://creativecommons.org/licenses/by/4.0/, CCR2-overexpressing mesenchymal stem cells targeting damaged liver enhance recovery of acute liver failure, Assessment of endothelial colony forming cells delivery routes in a murine model of critical limb threatening ischemia using an optimized cell tracking approach, Mesenchymal Stem Cells Promote Intestinal Mucosal Repair by Positively Regulating the Nrf2/Keap1/ARE Signaling Pathway in Acute Experimental Colitis, Cross Talk between Mesenchymal Stem/Stromal Cells and Innate Immunocytes Concerning Lupus Disease. Rodents. However, most of these studies were focus on its therapeutic effects or mechanisms. Treatment for peritoneal dialysis-associated peritonitis. We selected the day 3 as observation time point. In addition, the effects of different dosing modalities (e.g., oral administration and intraperitoneal injection) on the . and JavaScript. Thus, IP and AI routes are free of pulmonary embolism issue. The physiologic characteristic of the peritoneal cavity not only helps remove toxic metabolites from the body, but also provides a useful portal of entry in the body for several pharmacological agents. Easier to inject that intravenous. Subcutaneous Compared with Intraperitoneal Ketamine-Xylazine for While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. In vivo clearance of free DiR in healthy mice was showed in Supplemental Figure S4. prepared Figure 5. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Refractory Malignant Ascites. Real time-PCR and ELISA also demonstrate lower TNF- and higher IL-10, TSG-6 levels in IP group. ADS Ko, I. K. et al. Other gap junction blockers were also occasionally reported to . Further study demonstrated TSG-6 alone was sufficient to reduce intestinal inflammation in mice with colitis. Makhija, S. et al. Horses and other large animals: 1 mL per 10 lb of body weight, to a maximum . Blood 122, e23e32, doi: 10.1182/blood-2013-04-495119 (2013). (B) MSCs signal quantification of five main organs (lung, liver, spleen, kidney and large bowel) and corresponding MLNs. By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. We even tried 1108 cell/mouse in IP and AI group and no immediate death was occurred. Intraperitoneal Route of Drug Administration: Should it Be Used in In the meantime, to ensure continued support, we are displaying the site without styles 2021 Jul 23;2021:2303417. doi: 10.1155/2021/2303417. Google Scholar. Sala, E. et al. This work is licensed under a Creative Commons Attribution 4.0 International License. L.Z. Blonski, W., Buchner, A. M. & Lichtenstein, G. R. Treatment of ulcerative colitis. Clipboard, Search History, and several other advanced features are temporarily unavailable. Sci Rep 6, 30696 (2016). Insert the needle at a shallow angle through the . PubMed PubMed GTTs and ITTs in mice: simple tests, complex answers - Nature Article seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. and K.W. Article Muller-Ehmsen, J. ADS Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. Metabolite tracing, radioactive uptake experiments, and CRISPR screens demonstrate that FLVCR1 is the major plasma-membrane choline transporter. Lee et al. . Journal of Crohns & colitis 6 Suppl 2, S224S234, doi: 10.1016/s1873-9946(12)60502-9 (2012). A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. Analysis of tissues following mesenchymal stromal cell therapy in humans indicates limited long-term engraftment and no ectopic tissue formation. Parenteral administration routes include, but are not limited to epidermal, intraarterial, intramuscular (IM and depot IM) , intraperitoneal (IP) , intravenous (IV) , intrastemal injection or infusion techniques, intranasal (inhalation) , intrathecal, injection into the stomach, subcutaneous injections (subcutaneous (SQ and depot SQ . Since the IP injection induced more cells migration to the inflamed colon, we further compared the difference in mucosa healing of different delivery routes. and S.J. The expression level of TNF-, the most important pro-inflammatory cytokine, was markedly decreased in IP group, compared with AI (P=0.0174), IV (P=0.0019) and control group (P<0.001), respectively. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. The study overall defines appropriate doses and toxicity levels of dl1520 when injected intraperitoneally. Whole marrow cells are plated in the culture dish and non-adherent cells were removed during passage. 2010 May;27(5):735-8. doi: 10.1007/s11095-009-0031-z. The most efficient method to inject small animals is a two-person method where one holds the rodent and the other person injects the rodent at about 10 to 20 degrees in mice and 20 to 45 degrees in rats. and transmitted securely. Here, we combined the iDISCO + method and Fos immunostaining to confirm that the middle part of the right-side, but not the left-side, insular cortex in adult male mice is activated by intraperitoneal injection of lithium chloride. Incidence and phenotype of inflammatory bowel disease based on results from the Asia-pacific Crohns and colitis epidemiology study. Epub 2010 Mar 3. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane & Dittel, B. N. Isolation of mouse peritoneal cavity cells. Since that time, the literatures using stem cells for IBD have expanded. [20] There needs to be more research done to determine appropriate dosing and combinations of these drugs to advance intraperitoneal drug delivery. Interferon-gamma (IFN-gamma)- and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. Although controversial, intraperitoneal use in ovarian cancer has been recommended as a standard of care. Survival curve plots and Kaplan-Meier analyses were performed using Prism 5.0 software. Comparative analysis of chemokine receptors expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. The delivery of OK-432 occurred a total of four times in a span of one week. However, is IV injection the best way for MSCs delivery in IBD? analyzed the data, J.L. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. government site. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Intraperitoneal Drug Administration - an overview | ScienceDirect Topics In order to explore the possible mechanisms of these alterations, oxidative stress and inflammation levels in the mouse brain were examined. Kalchenko, V. et al. Intraperitoneal injections as an alternative method for micro-CT Cosnes, J., Gower-Rousseau, C., Seksik, P. & Cortot, A. During the observation, the body weight changes and fecal blood and their survival date was faithfully record. Further control study comparing bowel signals on healthy mice and DSS mice after MSCs injection showed weaker NIR signal on healthy bowel in all three routes, which indicated fewer MSCs migration to the colon without colitis (Supplementary Figure S5). The ELISA kit for TNFinduced protein 6 was used (CUSABIO Life science, China). Pentobarbital sodium Injection Caution. A similar increase in mouse cytokines in the peritoneum was seen after IP injections of human fibroblasts. Article Mice were under intensive observation for 12 days. Like pharmacological treatment, the cell dosing might also be an important issue for MSCs therapeutic success21. All fluorescent imaging procedures were conducted on small animal imaging system (IVIS Kinetics; Caliper Life Science). sharing sensitive information, make sure youre on a federal Intraperitoneal foreign bodies such as suture material, clips, and mesh also contribute to adhesion formation. Briefly, colonic segments were homogenized at 50mg/mL in phosphate buffer (50mM, pH 6.0) with 0.5% hexadecyltrimethylammonium bromide. Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy. [16] This study shows how intraperitoneal injections can be more effective and beneficial than other traditional routes of administration. MSCs has been well characterized of producing a range of modulatory cytokine, which inspires the designation of these cells as injury drugstore44. 2A). PubMed 8600 Rockville Pike Google Scholar. From the overall appearance, the hyperemia and edema of the colon in all MSCs treatment groups became milder than the DSS control. 4A,B). They found that PCs can directly migrated from peritoneal cavity and some of them were found at intestinal villi. In our study, we found inflamed colon could induce more MSCs engraftment than heathy colon, which partly supported this hypothesis, since inflamed colon has been reported of releasing variety of chemotactic cytokines26. DiR-labeled cells were injected into DSS mice by three different ways. [12] This study demonstrates how intraperitoneal injections can be used to help diagnose diseases by providing direct access to the peritoneal cavity and affecting the organs in the cavity. Thus, the aim of the current study was to find out the potential safety range of medical use. Stem cell transplantation showed promising results in IBD management. They found different source of MSCs with different delivery routes exhibited distinct effects on lung and cardiovascular injury. Pharm Res. Lu, Z., Wang, J., Wientjes, M. G. & Au, J. L. Intraperitoneal therapy for peritoneal cancer. In a case of a ruptured hepatocellular carcinoma, it was reported that the patient was treated successfully through the use of an intraperitoneal injection of OK-432, which is an immunomodulatory agent. Chemotactic cytokines might be involved and responsible for MSCs attraction. After 3 passages, spindle-shaped cell colonies gradually predominate in the cultures. This case is a good example of how IP injections can be used to deliver a drug that can help to treat or cure a medical diagnosis over the use of other routes of delivery. Although the decline in AGT activity in the liver was reversible, the activity remained at 75% of basal levels for up to 25 h after the final injection. [1] Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis. PDF Recommended Dose Volumes for Common Laboratory Animals - IQ Consortium Taede et al. Course of Crohns disease after allogeneic marrow transplantation. Methods of Euthanasia - Institutional Animal Care and Use Committee For example, if using a dose of 1 U kg -1 , a concentration of 0.2 U ml -1 is suitable. 5C). The levels of immunomodulatory cytokines were compared by real time-PCR and ELISA. (B) Quantification of % Ki-67+ cells/crypt, the IP and IA>IV>DSS control, n=6 mice/group; n=3 for the control group. Unlike MSCs, they are restricted by migration because of receptors in the peritoneal cavity specially recognizing the PCs29,30. . Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat. Tyndall, A. Factors such as peritoneal inflammation, surface area, peritoneal blood flow, time of contact, etc, influence the drug transfer. Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6. The incidence and impact of IBD is increasing worldwide. DSS only served as control. Sonazoid was utilized to aid with contrast-enhanced ultrasonography by enhancing the peritoneal and pleural cavities. Gastroenterology 145, 158165 e152, doi: 10.1053/j.gastro.2013.04.007 (2013). Gastric cancer is one of the most common malignant diseases worldwide [].Peritoneal dissemination is a characteristic feature of gastric cancer and is a critical factor underlying its poor prognosis [2-4].While clinical outcomes for gastric cancer patients with peritoneal dissemination have improved with advances in systemic and/or intraperitoneal (i.p) chemotherapy, desirable outcomes .

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